茶叶中儿茶素薄层色谱分离所用展开剂的优化.pdf

38 1 () Vol.38 No.1 2012 年 2 Journal of Hunan Agricultural University (Natural Sciences) Feb ．2012 DOI:10.34P.J.1238.2012.00102 茶叶中儿茶素薄层色谱分离所用展开剂的优化 a,b a,b a,b* b * ( a.b. 410128) 摘 要5 (992 – – 5.010.01.00.5 – – – 8.05.015.00.3 – – – 0.6 – – –0.6 – – –) – – 4 (EGCgEGCECgEC) 关 键 词 中图分类号S571.1 文献标志码A 文章编号1007− 1032(2012)01−0102−04 Optimization of developing solvents of high performance thin-layer chromatography for separating catechins in tea WANG Li-li a,b, WANG Kun-bo a,b, HUANG Jian-an a,b*, XU Zhong-xib* (a. National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals b.College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China) Abstract At room temperature, methylbenzene-acetone-formic acid (992, V/ V), methylbenzene-ethyl formate- methanol-formic acid (5.010.01.00.5, V/ V), methylbenzene-chloroform-acetone-formic acid (8.05.015.00.3, V/ V), chloroform-acetone-methanol-water (0.6, V/ V) and chloroform-acetone-formic acid-water (12.5 , V/ V) on silica gel marked as solvent Ⅰ, Ⅰ, Ⅲ , Ⅲand Ⅲrespectively were applied as developing solvent respectively in high performance thin-layer chromatography (HPTLC) for isolating catechins in the tea. Among them, solvent Ⅲ was found to be the best for separation of catechins, this solvent was then modified mainly regarding the volume ratios of formic acid. The results showed the modified