毕业论文--正常孕晚期女性碱性磷酸酶活性和血清蛋白水平的探讨.docxVIP

毕业论文（设计） 正常孕晚期女性碱性磷酸酶活性和血清蛋白水平的探讨 PAGE 2 PAGE 5 正常孕晚期女性碱性磷酸酶活性和血清蛋白水平的探讨 摘要：目的 观察女性孕晚期碱性磷酸酶（ALP）活性和血清蛋白水平的变化，以期为监测孕妇和胎儿的健康状况做到优生优育提供依据。方法 收集2014年8~2015年5月285名住院产妇的血清作为实验组，285名健康非孕育龄妇女（血糖、血脂、肝功能等生化指标均正常，无既往肝肾疾病、无高血压及其他内分泌系统疾病）的血清作为对照组，采用连续监测法检测两组标本的碱性磷酸酶的活性，双缩脲法检测总蛋白的水平，溴甲酚绿法检测白蛋白的水平，并收集检测数据进行比较。结果 孕晚期组血清ALP活性为189.65±45.32U/L，明显高于非妊娠组（78.93±25.62U/L），活性水平差异具有统计学意义（P0.01）。而孕晚期组总蛋白水平（54.3±3.5g/L）和白蛋白水平（30.4±3.6g/L）低于非妊娠组总蛋白水平（71.3±5.6g/L）和白蛋白水平（45.6±4.5g/L），蛋白水平差异具有统计学意义（P0.05）。结论 妊娠晚期女性血清ALP活性明显升高，血清蛋白水平偏低。晚期妊娠妇女血清中ALP含量，可作为胎儿生长发育和胎盘功能的动态监测指标。 关键词：碱性磷酸酶; 总蛋白; 白蛋白; 孕晚期 Research on the Changes of ALP Activity and the Level of Serum Protein in the Normal Late-stage Pregnant Women Abstract：Objective To provide a theoretical basis for Monitoring the health of pregnant women and fetal through investigating the activity changes of alkaline phosphatase(ALP) and serum protein in late-stage pregnant women. Methods The activities of ALP and the level of total protein and albumin were determined in 285 normal late-stage pregnant women (observation group) during August 2014 to May 2015 in maternity hospital . Control group is not pregnant women (blood glucose, blood lipid ,liver function and biochemical indexes is normal, without kidney disease, high blood pressure and other endocrine system diseases). Continuous monitoring method is used to test the activity of alkaline phosphatase, Biuret method to detect the level of total protein, Bromocresol green method to detect the level of albumin . Result Normal late-stage pregnant serum ALP（189.65±45.32U/L） is significantly higher than control group（78.93±25.62U/L）and has statistical significance (P0.01) . But the level of serum protein is slightly lower and has statistical significance(P0.05). Conclusion Normal pregnant women in late pregnant ,serum alkaline phosphatase increased significantly,Serum protein reduced slightly .Dynamic measurin