PKC对糖尿病肾病患者血小板的影响与临床意义.pdfVIP

Abstract The Influence of Protein Kinase C in Platelets of Diabetic Nephropathy Patients and Clinical Significance related The Influence of Protein Kinase C in Platelets of Diabetic Nephropathy Patients and Clinical Significance related Abstract Objective:To investigate the relationship between protein kinase C abnormalities and the low platelet count or the increase of platelet destruction in Diabetic Nephropathy patients. further more, to explain the clinical significance related. Methods:1.Experiment in vitro:Normal washed platelets were stimulated with phorbolmyristateacetate(PMA) or Staurosporine(STS) at 37 ℃.Platelet mitochondrial membrane potential or phosphatidylserine were monitored after stimulation with Flow Cytometry.Death-associated proteins(apoptosis proteins) were detected after stimulation by SDSand Protein Immunoblotting.2.Experiment in vivo:In 30 patients with diabetes mellitus and 34 patients with diabetic nephropathy, the variation of platelet mitochondrial membrane potential were measured by Flow Cytometry. Results:1.PMA induces the Depolarization of Mitochondrial Inner Transmembrane Potential (△ψm) and the increase of cleaved caspase3 in platelets.2.STS induces Depolarization of Mitochondrial Inner Transmembrane Potential ( △ψm) and Phosphatidylserine (PS) Exposure in platelets.Besides,STS disturbs balance of Bcl-xl/Bak Ratio in platelets.3.Gö6983,a specific inhibitor of PKC can not directly induce △ψm depolarization and PS exposure in platelet.Inhibition of ROS production attenuates the influence of STS in platelets.4. Depolarization of Mitochondrial Inner Transmembrane