高强度聚焦超声结合微泡造影剂影响原头蚴酶活性.doc

高强度聚焦超声结合微泡造影剂对原头蚴酶活性的影响? ?[基金项目]国家自然科学基金面上项目 通讯作者:叶彬，Tel:023E-mail:yebina@. 作者单位：1.重庆医科大学病原生物学教研室，重庆400016； 2.重庆医科大学分子医学与肿瘤研究中心，重庆400016； 3.青海省地方病防治研究所，青海811602； 4.重庆医科大学医学超声工程研究所，重庆400016。 孔 璟1，蔡辉1，叶 彬1,2?，张 静1,2，韩秀敏3，杨永海3，李发琪4 摘要：目的 探讨高强度聚焦超声结合适宜比例的微泡造影剂对离体细粒棘球绦虫原头蚴酶活性的影响。方法 在原头蚴悬液中加入比例为80︰1的微泡造影剂（含氟脂质体微泡），以声功率为50W的高强度聚焦超声波辐照30s。辐照后悬液涂片作酶组织化学染色，检测原头蚴三磷酸腺苷酶、葡萄糖-6-磷酸酶和琥珀酸脱氢酶的活性。结果 超声剂量相同时，微泡造影剂组的原头蚴三磷酸腺苷酶、葡萄糖-6-磷酸酶和琥珀酸脱氢酶活性明显低于单纯超声辐照组（P＜0.05）；阴性对照组无酶反应物产生。结论 高强度聚焦超声结合微泡造影剂能增强对离体原头蚴三磷酸腺苷酶、葡萄糖-6-磷酸酶和琥珀酸脱氢酶活性的抑制作用，这可能是其对原头蚴杀伤效应增强的原因之一。 关键词：包虫；原头蚴；高强度聚焦超声波；微泡造影剂；三磷酸腺苷酶；葡萄糖-6-磷酸酶；琥珀酸脱氢酶 中图分类号：R512.6 文献标识码：A The high intensity focused ultrasound with the aid of microbubble contrast agent decreased the enzymatic activity of Echinococcus granulosus protoscolices Kong Jing1, Cai Hui1，Ye Bin1,2*，Zhang Jing1,2，Han Xiuming3，Yang Yonghai3，LI Faqi4 1. Department of Pathogenic biology, Chongqing Medical University, Chongqing 400016, China 2. Research Center for Molecule Medicine and Tumor, Chongqing Medical University, Chongqing 400016, China； 3. Institute for Endemic Disease Control, Qinghai Province, Qinghai, 811602, China; 4. Institute of Ultrasound Engineering in Medicine, Chongqing Medical University, Chongqing 400016, China. ABSTRACT: Objective To investigate the effects of high intensity focused ultrasound (HIFU) with the aid of microbubble contrast agent (MBCA) on the enzymatic activities of E. granulosus in vitro. Methods The suspensions of protoscolices mixed with MBCA at the ratio of 80︰1 were treated with HIFU under the condition of constant parameter of fixed sound intensity (50 aoustic Watt) and treatment time (30 seconds). The activities of adenosine triphosphate (ATP), glucose-6-phosphatase (G-6-P) and succinate dehydrogenase (SDH) were investigated by the histochemical staining methods for enzymes. Results It was found that in the treated groups whose protoscolices suspensions mixed with the proportions of MBCA, the enzymatic act