酸敏感离子通道在外周化学感受器呼吸调节中的作用.doc

酸敏感离子通道对低O2及高CO2所致呼吸反应的影响 李丽1 刘文彦2 苗文静3 （1济宁医学院药学院，山东，日照，276826；2 济宁医学院基础学院，山东，济宁，272067；3 日照市妇幼保健院检验科，山东，日照，276826） 摘要：目的 研究大鼠吸入低O2和高CO2气体产生的呼吸效应，探讨酸敏感离子通道（acid sensing ion channal, ASICs）在此过程中的作用。方法 将健康成年SD大鼠40只随机分为生理盐水+空气对照组（n=8）、生理盐水+低O2组（n=8）、生理盐水+高CO2 组（n=8）、阿米洛利+低O2组（n=8）、阿米洛利+高CO2 组（n=8）。通过气管插管侧管吸入低O2和高CO2气体，以膈肌肌电为指标，记录低O2和高CO2引起的呼吸效应；通过脑室内预先注射阿米洛利，然后再吸入低O2和高CO2气体，观察脑室内微注射ASICs阻断剂阿米洛利对低O2和高CO2气体所致呼吸反应的影响。结果 对生理盐水+空气对照组大鼠，气管插管侧管吸入空气后呼吸较吸入空气前无明显变化（P〉0.05），脑室内预微注射生理盐水对呼吸没有产生明显影响（P〉0.05）。对生理盐水+低O2组大鼠，缺氧30min后，呼吸较缺氧前表现为明显的抑制（P0.01）；对阿米洛利+低O2组大鼠，脑室内预微注射阿米洛利后，缺氧后30min较缺氧前尚未表现为呼吸抑制（P〉0.05）。对生理盐水+高CO2 组大鼠，吸入高CO2气体1min，呼吸表现为明显的兴奋（P0.01）；脑室内预微注射阿米洛利后，呼吸兴奋的程度明显减弱（P0.01）。结论 ASICs参与了缺氧所致的呼吸抑制和高CO2用所致的呼吸兴奋过程的产生。 关键词ASICs; 阿米洛利； 呼吸调节； 低O2；高CO2 中图分类号:R743.3 文献标志码: A Effect of ASICs on the respiratory responses induced by hypoxia and inhalation of high concentrated CO2 (1College of pharmacy, Jining Medical University, Rizhao 276826, China; ?Academic College, Jining Medical University, Jining 272067, China; 3Clinical laboratory, Rizhao maternal and child health hospital, Rizhao 276826, China. ) Abstract: Objective To study the respiratory responses induced by hypoxia and inhalation of high concentrated CO2, and to dicuss the fuction of acid sensing ion channels(ASICs) in this process. Methods 40 healthy SD rats were divided into normal saline plus air control group(n=8) and normal saline plus hypoxia group(n=8) and normal saline plus high CO2 group(n=8) and amiloride plus hypoxia group(n=8) and amiloride plus high CO2 group(n=8). Hypoxic gas and high concentrated CO2 were inhaled by one side tube of tracheal intubation. The electromyogram (EMG) of diaphragm was monitored to observe the respiratory responses induced by hypoxia and high concentated CO2. ASICs blockers amiloride was injected into the ventricle firstly and hypoxic and high concectrated CO2 gas was inhaled following to observe the effect of ASICs on the respiratory regulation in this