酵母单杂交系统及其实验操作.pptVIP

Yeast One Hybrid System I. Introduction II. Yeast Molecular Techniques III. Constructing a Reporter Vector for One-Hybrid Analysis IV. Generating a cDNA Library V. Constructing Screening a One-Hybrid Library VI. Analyzing Positive Interactions I. Introduction One-Hybrid System provides an in vivo genetic assay used for isolating novel genes encoding proteins that bind to a target, cis-acting regulatory element Protein-DNA interaction The differences between yeast one two hybrid II. Yeast Molecular Techniques Yeast strains Yeast strains C. Mating type compatibilities Y187 (