氨基酸分析仪原理特点.ppt

Quality Analysis of Amino Acids: The Biochrom AAA Solution.氨基酸分析仪特点 17-19th September 2012, DKSH China, Shanghai Principles of Chromatographic separation色谱分离原理 Buffer flow Sample A+B A B Detector Ion Exchange Process 离子交换 Na+ SO3- Cation exchange resin 离子交换树脂 The resin consists of spherical beads of sulphonated polystyrene, cross linked with divinylbenzene. 树脂由磺化的苯乙烯二乙烯苯组成 Production of resin 树脂生产 Reaction of styrene and divinylbenzene while mixing under pressure and elevated temperatures with initiators 苯乙烯和二乙烯苯在一定压力下，随着升温相互作用 Separation and purification of polymer beads 分离和纯化聚合物 Sulphonation of polymer beads at elevated temperature for several hours 聚合物在升温情况下磺化几小时 Isolation of beads within required size range followed by extraction and cleaning 分离出要求尺寸范围的聚合物，然后洗脱，清洗 Resin variables 树脂 Particle size 颗粒尺寸 Smaller resin will allow greater resolution of peaks for the same flow rate and column length 在流速和柱长相同的情况下，树脂越小分离度越好 Degree of cross-linking 交联度 8 to 9 % Degree of sulphonation 磺化度 The exchange capacity of the resin increases with the degree of sulphonation 树脂的交换能力随着磺化度的增加而增加 Parameters affecting the separation影响分离的参数 Reaction mechanism 反应机理 Hydrindantin: needed in the reagent to prevent a side reaction that would reduce the amount of Ruhemann’s purple formed. 还原茚三酮：在反应试剂中用于阻止减少蓝紫色化合物形成的副反应 Simplified reaction between Ninhydrin and amino acids 茚三酮和氨基酸单一反应 The imino acids (proline and hydroxyproline) do not have free alpha-amino groups. Reaction with ninhydrin forms a bright yellow compound monitored at 440nm 亚氨酸（脯氨酸和羟脯氨酸）没有自由?-氨基，和茚三酮形成明黄色化合物，最大吸收440nm. Principles of amino acid analysis AAA User Training Course Principles of amino acid analysis AAA User Training Course Principles of amino acid analysis AAA User Training Course Principles of amino acid analysis AAA User Training Course