pMAL-p5x大肠杆菌表达载体说明.pdfVIP

pMAL-p5x 编号 载体名称 北京华越洋生物VECT5320 pMAL-p5x pMAL-p5x 载体基本信息 载体名称: pMAL-p5x 质粒类型: 大肠杆菌表达载体；蛋白表达与纯化 高拷贝/低拷贝: 低拷贝 启动子: tac 克隆方法: 多克隆位点，限制性内切酶 载体大小: 5752bp 5 测序引物及序列: -- 3 测序引物及序列: -- 载体标签: MBP tag 载体抗性: Ampicillin 筛选标记: -- 备注: -- 稳定性: -- 组成型: -- 病毒/非病毒: -- pMAL-p5x 载体质粒图谱和多克隆位点信息 pMAL-p5X-His Vector 载体简介 The vector pMAL-p5X is designed to produce maltose-binding protein (MBP) fusions, where the protein of interest can be cleaved from MBP with the specific protease Factor Xa. MBP fusions made with this vector include an N-terminal signal sequence, so the fusion protein is directed to the periplasm. The MBP has been engineered for tighter binding to amylose resin. A gene or open reading frame is inserted into a restriction site of the vector polylinker, in the same translational reading frame as the malE gene (encoding maltose-binding protein). The fusion protein thus produced can be purified by amylose affinity chromatography. The sequence coding for the four amino acids Ile-Glu-Gly-Arg is present just upstream of the XmnI site. This allows the protein of interest to be cleaved from maltose-binding protein with the specific protease Factor Xa. Fragments inserted in the XmnI site (cleaves GAAGG↓ATTTC) will produce a fusion protein that, after Factor Xa cleavage, contains no vector-derived residues on the protein of interest. pMAL-p5x