标准、规范、准则_MFHPB-01 Determination of Commercial Sterility and the Presence of Viable Microorganisms in Canned Foods_ MFHPB-01.pdf

Government of Canada Gouvernement du Canada HPB Method MFHPB-01 March 2001 HEALTH PRODUCTS AND FOOD BRANCH OTTAWA DETERMINATION OF COMMERCIAL STERILITY AND THE PRESENCE OF VIABLE MICROORGANISMS IN CANNED FOODS 1. APPLICATION This method is applicable to the detection of viable microorganisms in commercially sterile foods packaged in hermetically sealed containers to determine compliance with the requirements of Division 27 and Sections 4 and 7 of the Food and Drugs Act and pertinent sections of the Canada Agricultural Products Act, the Meat Inspection Act and the Fish Inspection Act. This method replaces MFHPB-25C dated March 1989. 2. PRINCIPLE This method employs the aseptic sampling of the contents of hermetically sealed foods and the subsequent inoculation of suitable media to determine the presence or absence of viable organisms. A portion of the product is mixed with specified media and incubated under specified conditions of time and temperature. It is assumed that each viable microorganism will multiply under these conditions and give rise to a visible colony in solid media or turbidity in liquid media. This method is also used to characterize such organisms as follows: - as being present in mixed or pure populations; - as being anaerobic, aerobic or facultative; - as being mesophilic or thermophilic; - as having coccal, bacillal or spiral morphology.