氧化应激与2型糖尿病患者发病程度的相关性探讨.docxVIP

氧化应激与2型糖尿病患者发病程度的 相关性探讨 陈松滕勇陈宝炳 杭州市第三人民医院检验科 摘要： 口的研究氧化应激与2型糖尿病患者发病程度的关系。方法通过电子自旋偶联 共振(ESR)技术检测各组血清活性氧(reactive oxygen species, ROS)水平, 分析2型糖尿病患者与止常人血清氧化应激水平差界，以及2型糖尿病患者间氧 化应激水平与发病程度之间的关系;通过实时荧光定量RT-PCR技术分析与氧化 应激水平相关的基因的表达水平，在基因层面分析氧化应激水平与2型糖尿病 患者发病程度Z间的关系。结果与正常组相比，2型糖尿病患者组ROS显著上 升(P0. 01),氧化应激相关基因乙醛还原酶1基因、谷胱甘肽过氧化物酶1 基因、B-丙氨酸丙酮酸转氨酶基因、含黄素单氧化酶5基因均显著下调 (P0.01)。结论2型糖尿病患者随着发病程度加深，空腹血糖水平上升，并伴 随着ROS含量的增加，最终导致氧化应激相关基因表达下调。 关键词： 空腹血糖;活性氧;氧化应激; 作者简介：陈松(1973-)，男，本科，主任技师，主要从事免疫学、分子生物 学检验研究。 收稿日期:2017-05-02 Correiation between oxidative stress and the incidence of type 2 diabetes mellitus CHEN Song TENG Yong CHEN Bao-bing Clinical Labora to ry, the Third PeopleLsHosoital of Hangzhou; Abstract： Objective To study the relationship between oxidative stress and the incidence of type 2 diabetes mel lit us. Methods The serum reactive oxygen species ( ROS) level was detected by means of electron spin resonance coupl ing ( ESR) , then the differences of serum oxidative stress level bctwccn paticnts with type 2 diabetes and nornial people and the relationship between the level of oxidative stress and the severity of type 2 diabetes were respectively analyzed. The expression level of oxidative stress-related genes was detected using real-time fluorescence quantitative ( RT-PCR) , and the relationship between the severity of type 2 diabctcs emd oxidativc stress level was analyzed at the genetic level. Results The ROS level significantly increased in type 2 diabetes group than the normal group ( P 0. 01) , and oxidative stress-related genes such as aldehyde reductase 1 gene, glutathione peroxidase 1 gene, 0 -alanine pyruvate transaminase gene, flavin-containing monooxygenase 5 gene signifiedntly decreased than the nornieil group ( P 0. 01) ? Conclusion The levels of fasting blood-glucose and ROS of patients of type 2 diabetes rise with the deterioration of disease, which leads to the downregulation expression of oxidative stress-related genes. Keyword： Fasting blood-glucose; Reactive oxy