抗心肌细胞损伤天然产物的发现与分析.pdfVIP

摘要 起的细胞凋亡作用，C20 和C40 抵抗PA 诱导的心肌凋亡可能与炎症信号通路相 关。 结论：1）绿原酸类似物CGA-6 抵抗H O 诱导的H9c2 心肌细胞损伤作用优 2 2 于绿原酸，其可能的机制是经其抗氧化应激参与的线粒体凋亡通路途径和抑制 JNK 及ERK 的激活减少细胞凋亡；2 ）获得4 个抵抗PA 诱导H9c2 心肌细胞脂 毒性损伤活性强于姜黄素的衍生物。 关键词： 氧化应激；心肌细胞凋亡；天然产物；线粒体；MAPK ；脂毒性 Abstract Abstract Objects: To obtain natural compounds or highly effective derivatives/analogues that protected cardiomyocytes from oxidative stress or lipotoxicity, and investigate their anti-apoptotic mechanisms. Methods: 1) H9c2 cells were treated with H O at different concentrations, cell 2 2 viability was tested by MTT to determine the most appropriate experiment concentration. 2) H9c2 cells were divided into 4 groups according to different treatments (control group, CGA-6 group, H O group, H O +CGA-6 group), cell 2 2 2 2 viability was assayed with MTT. The lactate dehydrogenase (LDH) release and catalase (CAT) activity measurement were performed according to kit protocols. The intracellular reactive oxygen species, Ca2+, mitochondrial membrane potential (MMP), were measured with CM-H DCFDA, Fluo-4 and JC-1 fluorescent probes, 2 respectively; The DNA was stained with Hoechst 33342 to observe cell apoptosis; Expression level of proteins related to mitochondrial apoptotic pathways and MAPKs were analyzed by western blot. 3) Time and dose-dependent palmitate-induced H9c2 cells viability were tested by MTT and the most appropriate experiment