灵芝多糖提取工艺研究.pdfVIP

Advances in Microbiology 微生物前沿, 2018, 7(1), 26-37 Published Online March 2018 in Hans. /journal/amb /10.12677/amb.2018.71004 Extraction Process of Ganoderma lucidum Polysaccharides Yuantao Yin, Qingzhi Yao* College of Life Science, Inner Mongolia Agriculture University, Huhhot Inner Mongolia th th rd Received: Mar. 5 , 2018; accepted: Mar. 16 , 2018; published: Mar. 23 , 2018 Abstract In this paper, the optimum extraction process of two kinds of Ganoderma lucidum polysaccharide was studied, and the content of two kinds of Ganoderma lucidum polysaccharide was compared. The hot water extraction method is used to study the conditions of extracting polysaccharides from Ganoderma lucidum. With taking the polysaccharide yield as the evaluation index, the single factor test and L18 (35) orthogonal test are used to examine the effect of the extraction ratio, pH value, extraction tem- perature, ethanol concentration and extraction time on the yield of Ganoderma lucidum polysaccha- rides. The optimum processing conditions for Ganoderma lucidum polysaccharides extraction with hot water are determined as that of extraction ratio 1:40, pH 7.5, extraction temperature 90˚C, extraction time 1.5 h, and ethanol concentration 90%. The content of Ganoderma lucidum polysaccharide was determined by Sevage method with the removal of free protein and 3,5-Dinitrosalicylic acid (DNS). Keywords Ganoderma, Polysaccharide, Extraction, Hot Water Extraction 灵芝多糖提取工艺研究 殷远滔，姚庆智* 内蒙古农业大学生命科学学院，内蒙古 呼和浩特 收稿日期：2018年3月5 日；录用日期：2018年3月16 日；发布日期：2018年3月23 日 摘 要 本文主要研究了两种灵芝多糖的最佳提取工艺，比较两种灵芝多糖含量的不同。采用热水浸提法对灵芝 *通讯作者。 文章引用: 殷远滔, 姚庆智. 灵芝多糖提取工艺研究[J]. 微生物前沿, 2018, 7(1): 26-37. DOI: 10.12677/amb.2018.71004