大孔吸附树脂富集酯型儿茶素.PDFVIP

68 2013, Vol.34, No.06 食品科学 ※工艺技术 大孔吸附树脂富集酯型儿茶素 1 2 2 2 1 1,* 黄 磊 ，岳鹏翔 ，郁 军 ，杨军国 ，陈 暄 ，黎星辉 (1.南京农业大学园艺学院，江苏 南京 210095 ；2.大闽国际集团研发中心，福建 漳州 363000) 摘  要：以绿茶提取物——茶粉为原料，研究采用大孔吸附树脂富集酯型儿茶素的工艺。通过比较4种树脂对酯型 儿茶素的静态吸附和解吸能力，筛选出最优树脂LX-16 ，并考察其静态吸附速率曲线、吸附等温线，以及动态吸附 与乙醇梯度洗脱等特性。结果表明：LX-16大孔吸附树脂对酯型儿茶素具有良好的吸附选择性，按酯型儿茶素上样 量17mg/g树脂上样，通过梯度洗脱，酯型儿茶素产品纯度从7.3%提高到了65.2%，回收率达80% 以上。 关键词：酯型儿茶素；大孔吸附树脂；吸附；解吸 Use of Macroporous Adsorption Resin for the Enrichment of Esterified Catechin 1 2 2 2 1 1,* HUANG Lei ，YUE Peng-xiang ，YU Jun ，YANG Jun-guo ，CHEN Xuan ，LI Xing-hui (1. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China； 2. Research and Development Center, Damin International Group, Zhangzhou 363000, China) Abstract ：Macroporous adsorption resin was used for the enrichment of esterified catechin from green tea powder. LX-16 was selected from four macroporous adsorption resins by evaluating adsorption and desorption capacity, and investigating velocity curve of static adsorption, adsorption isotherm, dynamic adsorption and alcohol gradient elution. The results showed that LX-16 revealed better adsorption selectivity for esterified catechins. When the loading amount of esterified catechin was 17 mg/g, the purity of purified esterified catechin was 65.2% as opposed to 7.3% for crude esterified catechin, and a recovery rate of 80% was obtained. Key words ：esterified catechin ；macroporous adsorption resin ；adsorption ；desorption 中图分类号：TS201.1 文献标志码：A 文章编号：1002-6630(2013)06-0068-05 研究表明，茶叶主要成分茶多酚(tea polyphenols ，