分水岭算法分割显微图像中重叠细胞.PDFVIP

11 12 Vo.l 11, No. 12 2006 12 Journal of Image and G raphics Dec. , 2006 丛培盛 孙建忠 (, 200092) , , , , , , C++ , , , , 768 76 AMD 1600+ CPU 2 s, , , : TP391. 41 Q233 : A : 2006) Application ofW atershed A lgorithm for Segm enting Overlapping Cells inM icroscopic Image CONG Peisheng, SUN Jianzhong (D ep artm ent of C hem istry, Tongj i University, Shangha i 200092) Abstract In order to realize autom atic and fast analysis ofm icroscopic mi age, in th is paper, the b inarized mi age was first transformed to distance form at. Fast grayscale reconstruction algorithm was then em ployed to reconstruct the transformed mi age. FinallyWatershed algorithm w as used to segm en t the result mi age. In thisway, the defect of thew atershed algorithm that the w atershed marker extracting often depends on prior know ledge of the mi age is avoid and the algorithm to be suitable for real clin ic practice and the au to segm entation of conglutination cells is realized. At last the algorithm w asprogrammedw ith C++ andw as used to segment the real clin ical fall off cell and pathology cell mi ages. A fter being applied to many d ifferent d isease and d ifferent background cell mi age, result show s that the algorithm can pick up destination cells in a given mi age and autom atically segm ent overlap cells accurately and rapidly under the cond ition of even illum ination. For a given 768 76 mi age, the algorithm can fin ish the segm entation w ith in 2 seconds in a AMD 1600+ CPU. And the segm entation resu lt is certificated by director doctor. Thus