低温保存对血小板凋亡的影响.pdfVIP

低温保存对血小板凋亡的影响 摘 要 目的 探讨低温保存对血小板凋亡的影响。 方法 将健康人洗涤血小板分别保存在 0℃、4 ℃、10℃、22 ℃及 37℃等 5 个温度共5 天。每天均对所有标本进行相关指标的检测。应用流式细胞技术检测 线粒体膜电位的改变。应用Western blot 检测血小板凋亡指标caspase-3 和Bcl-xl 的表达情况。 结果 流式细胞技术检测结果显示，保存在以上每一温度中的血小板均发生 线粒体膜电位去极化。与其他组相比，保存于10℃的血小板的线粒体膜电位去极 化受到明显抑制。Western blot 检测结果显示，当血小板保存于 10℃时，凋亡蛋 白caspase-3 的活化受到明显抑制，而抗凋亡蛋白Bcl-xl 表达未见明显下降。但是， 保存于其他温度中的血小板均出现凋亡蛋白 caspase-3 活化及抗凋亡蛋白 Bcl-xl 表达的下降。 结论 该实验的数据提示，低温保存对血小板凋亡起抑制作用。实验结果提 示我们有必要对保存在低温中的血小板的凋亡进行进一步的探讨。 关键词：血小板；凋亡；低温 1 Effection of Low Tempreture on Platelet Apoptosis Abstract Objective To survey the effection of low tempreture on platelet apoptosis. Methods Washed healthy people platelets were stored at 0℃,4℃,10℃,22℃ and 37℃ for up to 5 days. All samples were measured every day.Flow cytometry detected the change of mitochondrial membrane potential. Western blot analysis the apoptosis proteins caspase-3 and antiapoptosis proteins Bcl-xl express. Results The data from flow cytometry indicate that every group exists the change of depolarization of mitochondrial transmembrane potential ( △Ψm). Compared with other groups, the depolarizations of △Ψm of the group which was stored at 10 ℃ were inhibited significantly.When stored at 10 ℃,several apoptoic events in apoptosis including down-regulation of Bcl-xl and caspase-3 activation were inhibited significantly, according to the results of western blot.However, when platelets stored at the other groups, apoptosis protein caspase-3 were activated and expression of anti-apoptosis protein Bcl-xl were decreased. Conclusion These data suggest that low tempreture