白芍发酵菌种及发酵工艺的优选.PDFVIP

·2924 · 中草药 Chinese Traditional and Herbal Drugs 第 45 卷 第 20 期 2014 年 10 月 白芍发酵菌种及发酵工艺的优选 1 1 1 1 1 2* 于定荣 ，麻印莲 ，顾雪竹 ，陈 畅 ，程志铭 ，车 勇 1. 中国中医科学院中药研究所，北京 100700 2. 山东医药技师学院 制药工程系，山东 泰安 271000 摘 要：目的 优选白芍 Paeoniae Radix Alba 发酵菌种及发酵工艺条件。方法 以白芍与硫磺熏蒸白芍用乳酸菌和酵母菌进 行发酵，以活菌数为指标，综合芍药苷定量测定结果，优选白芍发酵菌种及发酵工艺条件。结果 白芍以酵母菌发酵效果为 佳；白芍和硫磺熏蒸白芍最佳发酵工艺均以酵母菌加培养基发酵 36 h 为佳。结论 白芍在发酵过程中，酵母菌发酵效果优 于乳酸菌发酵效果，优选的发酵工艺可行。 关键词：白芍；芍药苷；硫磺熏蒸；发酵；乳酸菌；酵母菌 中图分类号：R284.2 文献标志码：A 文章编号：0253 - 2670(2014)20 - 2924 - 04 DOI: 10.7501/j.issn.0253-2670.2014.20.010 Optimization of fermentation process and bacteria strain for Paeoniae Radix Alba 1 1 1 1 1 2 YU Ding-rong , MA Yin-lian , GU Xue-zhu , CHEN Chang , CHENG Zhi-ming , CHE Yong 1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China 2. Department of Pharmaceutical Engineering, Shandong Medicine Technician College, Tai’an 271000, China Abstract: Objective To optimize the fermentation process and bacteria strain for Paeoniae Radix Alba (PRA). Methods Both the crude drugs of PRA and PRA processed by sulfur fumigation were fermented with lactobacillus and microzyme. Using viable counts and paeoniflorin content as indexes, the fermentation process and bacteria strain for PRA were optimized. Results The microzyme had the best fermentation effect. The optimal fermentation process of PRA and sulfur fumigating PRA was for 36 h adding yeast and culture medium. Conclusion PRA can be fermented and the optimal fermentation process was for 36 h by adding microzyme and culture medium. Key words: Paeoniae Radix Alba ; paeoniflorin; sulfur fumigation; fermentation; lactobacillus; microzyme 白芍为毛茛科植物芍药Paeonia lactiflora Pall. 利用双向发酵法，观察白