独蒜兰种子共生萌发研究.PDFVIP

微生物学通报 Jun 20, 2008, 35(6): 909~912 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 研究报告 独蒜兰种子共生萌发研究 1,2 1* 1 杨友联 刘作易 朱国胜 (1. 550006) (2. 553004) 摘 要: 采用从独蒜兰分离的菌根真菌与其种子在燕麦培养基上共生萌发。结果表明GN21 、GN23 和GN24 的作用效果较好, 其中GN21 萌发率84.6%, 高于对照7 个百分点, 达到显著性。同时也 表明, 从同一兰科植物分离到的属于同一属的不同菌根真菌种, 有的能明显促进种子萌发, 有的 却不能促进萌发, 甚至产生病斑, 引起原球茎死亡。 关键词: 独蒜兰, 共生萌发, 菌根真菌 Study on Symbiotic Seed Germination of Pleione bulbocodioides (Franch) Rolfe YANG You-Lian1,2 LIU Zuo-Yi1* ZHU Guo-Sheng1 (1. Key Lab of Agricultural Biotechnology of Guizhou Province, Guizhou Academy of Agricultural Science, Huaxi 550006) (2. Department of Biology and Geography, Liupanshui Teachers ’ College, Shuicheng 553004) Abstract: The symbiotic seed germination of Pleione bulbocodioides (Franch.) Rolfe, a traditional Chi- nese medicine, was investigated. The results showed that the mycorrhizal fungi isolated from pleione bulbocodioides, GN21, GN23 and GN24 promoted the seed germination evidently, and the germination rate of GN21 was up to 84.6%, much better than the others and control. However, the fungal species isolated from the same species of orchid, even if belonging to the same genus, could not be always to stimulate the seed germination, some species even became pathogens under the circumstances of poor nutrition. Keywords: Pleione bulbocodioides, Symbiotic seed germination, Mycorrhizal fungus (Pleione bulbocodioides(Franch.)Rolfe) ,