fate of the bacterial cell envelope component,：细菌细胞膜成分的，.pdfVIP

Coastal Marine Science 32(3): 000–000, 2009 Fate of the bacterial cell envelope component, lipopolysaccharide, that is sequentially mediated by viruses and flagellates 1 2 3 1 4 Akira SHIBATA *, Hirohiko YASUI , Hideki FUKUDA , Hiroshi OGAWA , Tomohiko KIKUCHI , Tatsuki TODA2 and Satoru TAGUCHI2 1 Ocean Research Institute, The University of Tokyo, 1–15–1 Minamidai, Nakano, Tokyo, 164–8639, Japan 2 Faculty of Engineering, Soka University, 1–236 Tangi-cho, Hachioji, Tokyo 192–8577, Japan 3 Ocean reserch institute, University of Tokyo, Akahama, Otsuchi, Iwate 028–1102 Japan 4 Faculty of Education and Human Sciences, Yokohama National University, 792 Tokiwadai, Hodogaya, Yokohama 240–8501 Japan * E-mail: shibata@ori.u-tokyo.ac.jp Received: 18 September 2008; Accepted 10 Desember 2008 Abstract — Transformation of a cell envelope component of bacteria, lipopolysaccharide (LPS), from bacterial cells into a dis- solved form was investigated during time course experiments using seawater culture in conjunction with determining popula- tion dynamics of three microbial groups, which are flagellates, bacteria, and viruses. The surface seawater collected from a coastal site was enriched with glutamic acid, and then incubated. LPS concentration was determined using the Limulus amebo- cyte lysate assay. In the early period of incubation, LPS in the particulate fraction (0.2 m m) increased to the maximum in associ- ation with bacterial abundance; thereafter, both decreased together. During the fluctuation of LPS in the particulate fraction, LPS in the dissolved fraction (0.2 m m) also increased with viral abundance, but then de