巨胚米研究分析解析.docVIP

一个新巨胚等位基因的鉴定及性状考察与表达分析 平宝哲，时亚琼，谢米雪，张鑫，王鑫，张涛，李建粤基金项目： 基金项目：本研究由上海市科委项目(063919141) 资助 作者简介：平宝哲（1988-），男，硕士，研究方向：分子遗传学与基因工程。E-mail163.com *通信作者：李建粤，副教授，研究方向：分子遗传学与基因工程。E-mail: lijianyue01@ （上海师范大学 生命与环境科学学院，植物种质资源开发中心，上海200234） 摘要：“超2-10”水稻在离体培养过程中发生胚性状突变获得“上师大5号”巨胚水稻。本研究以“超2-10”水稻和“上师大5号”巨胚水稻为材料，分析了两2种水稻的胚重和、胚与糙米重量质量比、巨胚性状的遗传，并克隆和分析了其中巨胚基因（GE）序列。结果显示，（1）“上师大5号”的胚重及、胚与糙米重量质量比，都极显著大于“超2-10”水稻，“超2-10”水稻的胚重接近或大于一些已报道的巨胚水稻。（2）“上师大5号”水稻巨胚表型由一对隐性基因控制，并与韩国巨胚稻“M-2-565-11-3-B(ge)”属于相同的等位基因突变。（3）“超2-10”、“上师大5号”和“M-2-565-11-3-B(ge)”三3种水稻各自具有一个新的GE等位基因，它们的突变位点分别位于：只在启动子、启动子及编码链、只在编码链。（4）在“上师大5号”水稻中，启动子及编码链都有突变的GE等位基因（gec），在开花后4天至~6 天d，表达量呈现持续增加，完全不同于只有编码链突变的GE等位基因是呈持续下降的表达模式。本研究首次报道了突变只在启动子、突变同时发生在启动子及编码链 关键词：巨胚基因，；“上师大5号”水稻，；“超2-10”水稻，；“M-2-565-11-3-B(ge)”水稻，；新等位基因，；表达分析 全文口语化表达，不够精炼。建议科学严谨表达研究结果。 Molecular Characterization, Phenotype Investigation and Expression Analysis of a New Giant Embryo Allele PING Baozhe, SHI Yaqiong, XIE Mixue, ZHANG Xin, WAN Xin, ZHANG Tao, LI Jianyue* （Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, People’s Republic of China） Abstract: “Shangshida No. 5” is a new variety of giant embryo rice derived from embryo character mutation in “Chao2-10” rice tissue culture operation. In this paper, we use “Chao2-10” and “Shangshida No. 5” rice as materials. The embryo weight and ratio of embryo to brown rice of “Shangshida No. 5” rice were compared with those of “Chao2-10” rice. Then we did the genetic analysis of giant embryo phenotype. Giant embryo gene (GE) of “Shangshida No. 5” rice, “Chao2-10” rice and Korea giant embryo rice“M-2-565-11-3-B (ge)” were cloned to analyse their sequence. The expression levels of GE gene in “Shangshida No. 5” rice, “Chao2-10” rice were analysed by Real time-PCR with the “Nipponbare” rice as a control. The results were as follows: (1) Both embryo weight and ratio of embryo weight to brown rice weight of “Shangshida No. 5” rice w