超声波提取野菊花总黄酮及含量测定.doc

PAGE PAGE 毕 业 设 计（论文） 2011 届 题 目 超声波提取野菊花 总黄酮及含量测定 专 业 制药工程 学生姓名 李明艳 学 号 指导教师 肖 莉 论文字数 完成日期 20 年 月 日 湖 州 师 范 学 院 教 务 处 印 湖州师范学院学士学位论文 PAGE 超声波提取野菊花总黄酮及含量测定 李明艳 （湖州师范学院生命科学学院，湖州 313000） 摘要：本实验主要利用超声波提取技术提取野菊花中的总黄酮，用有机溶剂乙醇提取野菊花总黄酮的工艺路线，对所提取的总黄酮进行验证，并用分光光度计法测定其含量。在单因素提取的基础上，采取正交优化试验，探讨乙醇体积分数、液料比、提取时间对野菊花总黄酮提取工艺的影响，结果表明，影响野菊花总黄酮超声提取的主要因素为提取时间，其次为液料比、乙醇体积分数。最佳提取工艺条件为乙醇体积分数60％，液料比25:1，提取时间80min，测得野菊花总黄酮为5.62%。在优选的工艺条件下进行提取，并利用紫外可见分光光度法对野菊花总黄酮的含量进行测定。该提取工艺简单，易操作。 关键词：野菊花，总黄酮，超声波，提取 Extraction of total flavonoids from chrysanthemum by ultrasonic and content determination Li MingYan （School of Life Science，Huzhou Teachers College，Huzhou 313000 ） Abstract：This experiment using ultrasonic technology to extract the flavonoid in wild chrysanthemum. This process route is using organic solvent of ethanol to extract the flavonoids in wild chrysanthemum, to verify the extracting flavonoids, and its content determined by spectrophtometry. On the basis of single factor extraction orthogonal test be taken. The optimum extraction parameters of total flavonoids from Chrysanthemum indicum by ultrasonic were investigated including, the ratio of liquid and material, extraction time．It was found that the orders of parameters affecting extraction efficiency were extraction time，the ratio of material and liquid and alcohol content. The optimal extraction conditions were obtained as followed：alcohol content 60％, the ratio of liquid and material 25:1 and time 80 min．Under these conditions, the content of flavonoids reached 5.62％. Extraction in the optimal process conditions, and used the Ultraviolet spectrophotometry for determination the content of the flavonoid in wild chrysanthemum. The extraction process is simple and easy to operate . Key words：chrysanthemum indicum；total flavonoids；ultrasonic；extraction 目 录 TOC \o 1-3 \h \z \u 1 引言 1