作物淀粉的三维荧光光谱指纹分析鉴定方法.PDFVIP

第 41 卷 第 3 期 光 学 仪 器 Vol. 41，No. 3 2019 年 6 月 OPTICAL INSTRUMENTS June，2019 文章编号：1005-5630(2019)03-0042-07 DOI: 10.3969/j.issn.1005-5630.2019.03.007 作物淀粉的三维荧光光谱指纹 分析鉴定方法 张晋丹，吴明伟，冯 旻 （中国科学院 植物研究所，北京 100093 ） 摘要：采用淀粉固体粉末进行荧光光谱测定，并对三维荧光光谱(EEMs) 进行平行因子分析 (PARAFAC) ，研究了水稻、高粱、玉米等单子叶作物淀粉的荧光组分特征，探讨了荧光组分 特征与水稻品种的相关性。结果表明：不同来源的淀粉具有不同的荧光特性，可作为该物种 淀粉的指纹图谱；籼稻荧光组分的荧光强度明显低于粳稻，经过单因素方差分析，可确定籼 稻和粳稻的淀粉荧光组分强度具有显著差异，说明该方法可用于籼粳稻淀粉的鉴别。 关键词：淀粉；三维荧光光谱；平行因子分析；指纹图谱 中图分类号：O657.3; Q539+.1 文献标志码：A Three-dimensional fluorescence spectrum fingerprinting analysis method for crop starch ZHANG Jindan ，WU Mingwei ，FENG Min （Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China ） Abstract: This paper conducted fluorescent spectra of starch powder by using three dimensional fluorescence spectroscopy (EEMs) combined with parallel factors analysis (PARAFAC), revealing that different fluorescence features in the different crops. We built fluorescence fingerprint of several monocots seed starch such as rice, sorghum and maize. Furthermore, we tested the correlations of fluorescence properties and rice variety. The results indicated that fluorescence components intensity were lower in indica varieties than that in japonica varieties. One-way analysis of variance test showed statistic differences between indica varieties and japonica varieties. T