细胞外信号调节激酶在实验性脑缺血损伤皮层区的动态时空变化.docVIP

细胞外信号调节激酶在实验性脑缺血损伤皮层区的动态时空变化 102虫国鱼壅芏塑垒芏苤查!生旦蔓鲞蔓塑!r0ilamp;Neurol2007,V.I.14,N0.2 细胞外信号调节激酶在实验性脑缺血损伤 皮层区的动态时空变化 杨冀萍,刘怀军,张聪敏,王藏海,刘增品,李林芳 (河北医科大学第二医院,l,医学影像科,2,呼吸内科,河北石家庄050000) 摘要:目的探讨细胞外信号调节激酶(ERK)在局灶性脑缺fin/再灌注损伤梗死灶周皮层区的动态时空变化及 其作用机制.方法建立兔大脑中动脉阻断(MCAO)局灶性脑缺血再灌注模型,应用免疫组化检测ERK1在脑 缺血2h再灌注不同时间灶周皮层的动态表达规律,同时应用免疫组化和流式细胞术检测细胞凋亡状态的动态 变化.结果免疫组化分析显示,缺血2h再灌注灶周皮层区1hERK1表达开始增多,6h数目明显增多,3d 达高峰,然后逐渐下降.14d回落到基线水平,其阳性细胞数分别为0.22±0.02,0.25±0.02,0.42±0.04,0.14 ±O.02.其表达时程变化与灶周皮层凋亡的变化相一致.结论脑缺血损伤诱导ERK表达增强,ERK在介导 神经细胞凋亡和缺血性损伤中起重要作用.这为脑缺血治疗提供了新的思路. 关键词:脑缺血再灌注;细胞外信号调节激酶;凋亡 中图分类号:R743文献标识码:A文章编号:1006—2963(2007)02—0102—03 TemporospatialAlterationsofActivationofExtracellarSignal—regulated KinasesinCortexInvolvedintheInjuryofCerebralIschemia YANGJi—ping,LIUHuai—jun,ZHANGCong—min., WANGCang-hai.IIUZeng—pin.LILin—fang (1,DepartmentofMedicineImaging,2,DepartmentofRespiratoryMedicine. TheSecondHospitalofHebeiMedicalUniversity,HebeiShijiazhuang050000.China) ABSTRACT:ObjectiveToinvestigatethetemporospatialalterationsandeffectoftheactivationofextracellular signal—regulatedkinase1(ERK1)inperifocalcortexinvolvedincerebralischemici~ury.MethodsThecerebral ischemiafocally2handreperfusion72hmodelinrabbitswasinducedbytransientocclusionofmiddlecerebral artery(MCA).HerethedynamicchangesofexpressionofERK1inperifocalcortexoftherabbitbrainexposed to2hischemiaanddifferentperiodsofreperfusionweredetectedbyimmunohistochemistry.Meantime,the apoptosisstatusweredetectedbyimmunohistochemistryandbyflowcytometrymethods.ResultsERK1 activationwasfirstincreasedinperifocalcortexatlhofreperfusionandstronglyactivatedat6hwithapeakat 3dbyimmuohistochemistry.andgraduallydecreasedtobasallevelat14dofreperfusion(O.22±0.02,0.25± 0.02,0.42±0.04,0.14±0.02).Allthesewerecorrelatedwiththechangeofapoptosisintheperifocalcortex. ConclusionsERKsignalingpathwaywasinvolvedintheischemicinjuryandplayedanimportantroleinapoptosis ofneuronsandfocallycerebralischemia,thisfindingmayprovideatherapeuticapporachforcerebralischemia. Keywords:cerebralische