蛋白质类药物析课件.ppt

Multiply charged ion peaks of myoglobin analyzed by ESI-ion trap mass spectrometer （六）二级结构测定 通常采用圆二色光谱(CD）测定溶液状态下的蛋白质二级结构含量。 mdeg Wavelength (nm) 200 220 The far-UV CD spectra were obtained before and after 276 nm light continuous exc. (0.5 h, 1 h, 1.5 h, 2.5 h, 3.5 h, and 7 h) of insulin in solution. There is a progressive loss of ellipticity signal with 276 nm exc. time at 195, 209 and 222 nm. Circular dichroism spectrometry in the ultraviolet wavelength region (UV-CD) of MBP-cytochrome b fusion protein in different detergent solutions. （七）二硫键定位 Theoretical S. aureus protease fragmentation