Quantitative real-time PCR approaches for microbial community studies in wastewater treatment systems Applications and considerations 废水处理系统中微生物群落定量实时pcr方法的应用与思考.pdfVIP

Biotechnology Advances 31 (2013) 1358–1373 Contents lists available at ScienceDirect Biotechnology Advances journal homepage: www. elsevier. com/ locate/biotec hadv Research review paper Quantitative real-time PCR approaches for microbial community studies in wastewater treatment systems: Applications and considerations Jaai Kim a, Juntaek Lim b, Changsoo Lee a,⁎ a School of Urban and Environmental Engineering, Ulsan National Institute of Science and Technology (UNIST), 50 UNIST-gil, Eonyang-eup, Ulsan 689-798, Republic of Korea b Corporate Technology Division, POSCO, 440 Teheran-ro, Gangnam-gu, Seoul 135-777, Republic of Korea a r t i c l e i n f o a b s t r a c t Article history: Quantitative real-time PCR (qPCR) has been widely used in recent environmental microbial ecology studies as a Received 28 January 2013 tool for detecting and quantifying microorganisms of interest, which aids in better understandings of the com- Received in revised form 2 May 2013 plexity of wastewater microbial communities. Although qPCR can be used to provide more specific and accurate Accepted 28 May 2013 quantification than other molecular techniques, it does have limitations that must be considered when applying it Available online 6 June 2013 in practice. This article reviews the principle of qPCR quantification and its applications to microbial ecology stud-