ABR反应器处理苯胺黑药废水及其素微生物种群结构研究-环境工程专业毕业论文.docxVIP

广东工业大学硕士学位论文(5)对ABR反应器第1、2隔室污泥进行高通量测序，分别收获35003和 广东工业大学硕士学位论文 (5)对ABR反应器第1、2隔室污泥进行高通量测序，分别收获35003和 35632个有效序列，优化后进行归类操作，第一二两个隔室的样品覆盖度 (Coverage)分别为90．52％和89．68％，样品测序合理，能够真实反映反应器中的微 生物群落结构情况。在反应器第1格和第2格内，绿弯菌门菌群所占比重最大(分 别为24．73％和33．60％)，其次是厚壁菌门(分别为24．17％和20．92％)和变形菌门 (分别为1 8．93％和20．88％)。其它优势菌群有嗜热丝菌门、拟杆菌门、螺旋菌门、 浮霉菌门、疣微菌门、酸杆菌门、广古菌门、芽单胞菌门和绿菌门。在属的水平检 测到Caldisericum，Leptolinea，Leuconostoc和Bacillus等，这与反应器较好的 耐冲击负荷和降解苯胺黑药作用相一致。 关键词：ABR反应器；苯胺黑药；颗粒污泥；Miseq高通量测序 II 万方数据 ABSTRACTAB ABSTRACT AB STRACT Aniline aerofloat is a common floatation wastewater which brings more difficult to the treatment of Ore dressing wastewater with hard degradable for toxic and harmful．As a high-rate anaerobic reactor，Anaerobic Baffled Reactor has excellent ability to remove organic pollutants．An ABR reactor was set up and sludge domestication inoculated anaerobic tank sewage treatment plant sludge，the sludge concentration was adjusted to 2 1 560 mg／L．Hydraulic Retention Time was 24 h，a steady degradation of aniline aerofloat and COD removal situation with the operation of HRT、pH and other experimental factors external carbon ratio，and studies the properties and granular sludge formation mechanism．High-throughput sequencing technology was used to analysis community structure within the stable operation of the reactor microbial．The main conclusions are as follows： (1)A fixed HRT and increased volume loading was used to startup ABR． Gradient sludge acclimation nigrosine by increasing aniline aerofloat concentration． After 1 00 day’S running，domestication stable，the influent carbon source aniline aerofloat reached 250 mg／L，the degradation rate was 70．00％and remained stable， the lowest concentration of the effluent was 72．95 mg／L，the degradation efficiency reached 70．22％．The reactor was started successfully，the frontend compartments played major roles on removal of organic．The effluent VFA content was 200 mg／L or less，pH was 7．0，the system was not acidification． (2)HRT，pH，and external carbon degradatio