铁皮石斛促分裂原活化蛋白激酶基因DoMPK4的分离和差异表达_英文_.pdfVIP

·1076 · 药学学报 Acta Pharmaceutica Sinica 2014, 49 (7): 1076−1083 Isolation and differential expression of a novel MAP kinase gene DoMPK4 in Dendrobium officinale 1, 2 1 1 2 2* ZHANG Gang , LI Yi-min , HU Ben-xiang , ZHANG Da-wei , GUO Shun-xing (1. College of Pharmacy, Shaanxi Provincial Key Laboratory for Chinese Medicine Basis New Drugs Research, Shaanxi University of Chinese Medicine, Xi’an 712046, China; 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China) Abstract : Mitogen-activated protein kinases (MAPKs) are important signaling transduction components well conserved in eukaryotes and play essential roles in various physiological, developmental and hormonal responses in plant. In the present study, a MAPK gene, designated as DoMPK4 (GenBank accession No. JX297597), is identified from a rare endangered medicinal orchid species D. officinale using the reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods. The full length cDNA of DoMPK4 is 1 518 bp in length and encoded a 369 aa protein with a molecular weight of 42.42 kD and an isoelectric point of 5.55. DoMPK4 protein contained a serine/threonine protein kinase active site (158−170), a MAP kinase site (71−174), and eight conserved motifs. DoMPK4 had a transmembrane (214−232) but no signal peptide. Multiple sequence alignment showed that DoMPK4 shared