发酵培养中葡萄糖对超螺旋质粒DNA产量的影响.pdf

2005 年 1 月 　　 　化 　学 　工 　业 　与 　工 　程 第 22 卷 　第 1 期 　 J an . 2005 CHEMICAL 　INDUSTRY　AND 　EN GINEERIN G Vol . 22 　　No . 1 ( ) 　　文章编号 :1004 - 9533 2005 01 - 0040 - 04 发酵培养中葡萄糖对超螺旋质粒 D NA 产量的影响 1 2 2 徐英黔 ,吴 　蕾 ,甘一如 ( 1. 鞍山科技大学 化工学院 ,辽宁 鞍山 114002 ;2 天津大学 化工学院 ,天津 300072) 摘要 :本文首先研究了含有不同质量浓度葡萄糖的发酵培养基对超螺旋质粒 DNA 产量的影响 。结 果表明 ,发酵培养基中最佳葡萄糖的质量浓度为 200 gL 。此培养基与未添加葡萄糖的培养基相 比,超螺旋质粒DNA 产量提高256 倍 ;菌体密度提高 126 倍 。其次研究了优化培养基下影响超螺 旋质粒 DNA 产量的各参数变化规律 。 关键词 :超螺旋质粒 DNA ;发酵 ;葡萄糖 中图分类号 : R284 　文献标识码 :A Effect of Glucose on Supercoiled Plasmid D NA Production in Fermentation Process 1 2 2 XU Yingqian ,WU Lei , GAN Yiru ( 1. School of Chemical Engineering ,Anshan University of Science Technology ,Liaoning Anshan 114002 ,China ; 2 . School of Chemical Engineering ,Tianj in University ,Tianj in 300072 ,China) Abstract : In this paper the influence of glucose on the production of supercoiled plasmid DNA in the fermentation process is presented . Glucose of different concentration (0 gL , 1. 00 gL ,2 . 00 gL ,4 . 00 gL) was used as carbon source . The optimal concentration of glucose in culture medium was 2 . 00 gL . Supercoiled plasmid DNA concentration was enhanced by 2 . 56 and biomass concentration was enhanced by 1. 26 in comparison with those without glucose supplement in the optimized culture medium. Factors influenci