用DAPI进行细胞染色.docx

用DAPI进行细胞染色 DAPI的配置 Stock solution of 1mg/ml in ddH2O; working solution of 0.25mg/ml to 50mg/ml in ddH2O or PGM. stocked at -20 in the dark 1.Place sample on slide. 2.Add a few drops working solution. 3.Squash and view. Staining ? DAPI Staining for Cells: Separately DAPI staining: ? Wash the cells twice with PBS ? Incubate with 1: 750 of stock DAPI for 5 min in dark ? Wash 2 x 5 min with PBS in dark ? Rinse with water for a few seconds ? Air dry at RT in dark ? Mount with antifade solution (Vectashield, Co#: H-1000, Vector Lab) 5 μl for each d12 mm coverslip, 25 μl for square coverslip and