低强度红激光局部照射影响血管损伤后重塑的实验研究.docVIP

???? 低强度红激光局部照射影响血管损伤后重塑的实验研究 ???? ????【摘要】　目的　评价低能量红激光局部照射(LPRLI)对血管损伤后重塑模式的影响。方法　雄性新西兰白兔100只，随机分为球囊拉伤组和球囊拉伤加激光照射组。球囊拉伤右髂动脉后，应用激光球囊照射拉伤局部，总的能量密度为0.9 J/cm2。分别于术后3天、1周、2周、4周和8周分批处死动物，原位固定后截取病变血管进行病理组织学和免疫组织化学检测。最后1批动物在取材前进行髂动脉造影。结果　拉伤后3天单纯拉伤组管腔横截面积和外弹力板围绕面积明显低于激光照射组，管腔狭窄程度显著高于激光照射组(P＜0.05)；单纯拉伤组术后2周血管腔横截面积和外弹力膜围绕面积与术后3天相比有所增大(P＜0.05)，血管狭窄程度减轻(P＜0.05)，术后2～8周血管腔横截面积和外弹力膜围绕面积均进行性减小，狭窄程度逐渐加重(P＜0.05)。在8周时已显著小于正常血管外弹力膜围绕面积(P＜0.05)。激光组术后2～8周血管狭窄程度无明显变化(P＞0.05)，外弹力膜围绕面积进行性增大，到术后8周已显著大于正常血管的外弹力板围绕面积(P＜0.05)。术后1周内膜加中膜面积两组均出现明显增厚(P＜0.05)，从第2周开始，激光组内膜加中膜面积显著小于单纯拉伤组(P＜0.05)。髂动脉造影结果显示，单纯拉伤组血管最小内径明显小于激光照射组(P＜0.01)；激光照射后血管狭窄程度显著减轻(P＜0.01)。结论　激光局部照射可以显著改善血管损伤后重塑的建立模式，减少狭窄的发生。　　【关键词】　低强度红激光　再狭窄　重塑 The role of low power red laser illumination on remodeling after arterial injury in normal rabbits Li Guang, Chen Jiyan, Zhou Yingling, et al.（Guangdong Provincial Cardiovascular Institute, Guangzhou 510100） 　　【Abstract】　Objective　To investigate the effects of a certain density illumination of low power red laser on the remodeling after arterial injury. Method　One hundred male New Zealand white rabbits were randomized into two groups: balloon injury group (control group) and balloon injury followed by laser illumination group (treatment group: total illumination density:0.9 J/cm2). To study the time course of morphological changes, all the animals were killed on 3,7,14,28 or 56 days respectively after the procedure and with local fixation the iliac arteries were harvested for further pathomorphometrical analysis. At the end of 56 days, animals received angiography before killed. Results　Under microscope, the scores of iliac arterial injury were similar between the two groups( P＞0.05). On the third day after the procedure, recoils were seen in both groups, but the stenosis rate of balloon group were significantly higher than those of laser group (P＜0.05). The areas embayed by extral elastic membrane were altered synchronously with the lumen areas and it was smaller of balloon group than