排卵前期卵泡颗粒细胞端粒酶的表达及其影响因素.PDF

714 Acta Physiologica Sinica, December 25, 2005, 57 (6): 714-718 1 2,* 2 1 2 330006 (telomeric repeat amplification protocol-enzyme linked immunoadsordent assay, T R AP -E LIS A) (radioimmunoassay, RIA)(estradiol, E )(progesterone, P )MTT() 2 0 (human chorionic gonadotropin, HCG)(follicle-stimu- lating hormone, FSH)(dbcAMP)(verapamil)c-myb RIA verapamil FSH E2 P0 dbcAMP HCG c-myb E2 P 0 MTT hTERT FSH HCG verapamildbcAMP , Q492.5; R335+ .5 Telomerase: the expression and regulatory mechanisms in preovulatory ovarian granulosa cells 1 2,* 2 ZHANG Jing , ZHENG Yue-Hui , ZHENG Li-Ping 1 2 Center of Reproduction; Department of Physiology, School of Medicine, Nanchang University, Nanchang 330006, China Abstract: The objective of this study was to analyze the expression of telomerase in granulosa cells and the influential factors in telomerase expression. TRAP-ELISA (telomeric repeat amplification protocol-enzyme linked immunoadsordent assay) method was used to study the expression and control of telomerase in rat preovulatory ovary. We also used radioimmunoassay (RIA) to determine the expression of estradiol (E ) and progesterone (P ). Furthermore we used MTT to study the proliferation of ovarian granulosa cells. Telomerase activity 2 0 was significantly enhanced by human chorionic gonadotropin (HCG), follicle-stimulating hormone (FSH),