The changes of miRNA expression in rat hippocampus following chronic lead exposure 慢性铅暴露对大鼠海马mirna表达的影响.pdf

Toxicology Letters 229 (2014) 158–166 Contents lists available at ScienceDirect Toxicology Letters journal homepage: www.elsev /locate/toxlet The changes of miRNA expression in rat hippocampus following chronic lead exposure Jun An 1,2, Tongjian Cai 1, Honglei Che 1, Tao Yu, Zipeng Cao, Xinqin Liu, Fang Zhao, Jinfei Jing, Xuefeng Shen, Mingchao Liu, Kejun Du, Jingyuan Chen **, Wenjing Luo * Department of Occupational Environmental Health and the Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, 169 West Changle Road, Xi ’an 710032, China H I G H L I G H T S Lead exposure resulted in the changed expression of 7 miRNAs in rat hippocampus. The target genes of up-regulated miRNAs were related to neurophysiological pathways and neurodegenerative diseases. The changed miRNAs may play important roles in lead-induced neurotoxicity. A R T I C L E I N F O A B S T R A C T Article history: miRNAs have been found to contribute to normal brain functions, nervous system diseases, as well as Received 18 January 2014 neurotoxicities induced by external agents. However, whether they are involved in lead-induced Received in revised form 24 May 2014 neurotoxicities is still not clear. To identify that, a lead-induced chronic neurotoxicity model of rats was Accepted 1 June 2014 built. Both miRNA microarray analysis and qRT-PCR were performed to determine the ch