桑不同药用部位中黄酮类成分的定量检测.PDF

桑不同药用部位中黄酮类成分的定量检测 ＊ # 杨 晶 ，刘嘉琪，王宝昌，史丽颖，唐 玲，王永奇 （大连大学药物研究所，辽宁大连 116622） 中图分类号 R284.1；R927.2 文献标志码 A 文章编号 1001-0408（2014）27-2550-03 DOI 10.6039/j.issn. 1001-0408.2014.27.18 摘 要 目的：测定桑叶、桑椹、桑枝、桑白皮中总黄酮的含量。方法：以桑辛素为对照品，采用分光光度法测定桑叶、桑椹、桑枝、 桑白皮95％乙醇提取物及乙醇提取后水提取物中总黄酮的含量。结果：桑辛素的质量浓度在0.025～0.050mg/ml 范围内与吸光 度呈良好的线性关系（r ＝0.9998）；精密度、稳定性、重复性试验的RSD ＜1％。桑叶、桑椹、桑枝、桑白皮95％乙醇提取物中总黄酮 的质量分数分别为13.60％、7.19％、10.53％、18.38％，乙醇提取后水提取物中总黄酮的质量分数分别为0.26％、0.60％、0.40％、 0.01％。结论：桑白皮中黄酮类成分质量分数最高，有效部分为乙醇提取物。 关键词 桑；不同部位；总黄酮；含量测定；桑辛素；桑白皮 Quantitative Analysis of Flavonoids in Different Medicinal Parts of Morus alba YANG Jing ，LIU Jia-qi ，WANG Bao-chang ，SHI Li-ying ，TANG Ling ，WANG Yong-qi （Institute of Materia Medi- ca ，Dalian University ，Liaoning Dalian 116622，China ） ABSTRACT OBJECTIVE ：To determine the contents of total flavonoids in leaves ，fruit ，branch and root bark of Morus alba . METHODS ：With moracin as substance control ，the contents of total flavonoids in 95％ethanol extract ，water extract after ethanol extraction from the leaves ，fruit ，branch and root bark of M. alba were measured by spectrophotometry. RESULTS ：The linear range of moracin were 0.025-0.050 mg/ml （r ＝0.999 8）. RSD of precision ，stability and reproducibility tests were all lower than 1％. The mass fraction of total flavonoids in 95％ ethanol extract from the leaves ，fruit ，branch and root bark of M. alba were 13.60％，7.19％，10.53％ and 18.38％. The mass fraction of total flavonoids in water extract after ethanol extraction from the leaves ，fruit ，branch and root bark of M. alba were 0.26％，0.60％，0.40％and 0.01％. CONCLUSIONS ：The content of total fla- vonoids in root bark of M. alba is the highest ，ethanol extract of it is effective. KEYWORDS Morus alba ；Different parts ；Total flavonoids ；Content determination ；Moracin ；Root bark of M. alba 桑Morus