小鼠canstatincDNA的克隆及其在大肠杆菌中的-中国病理生理杂志.pdfVIP

( 998 ( Chinese Journal of Pathophysiology 200 , 20( 6) : 998- 1002 [ ] 1000- 718(200 ) 06- 0998- 05 canstatin cDNA* 侯卫红, 袁保梅, 王天云, 柴玉荣, 侯桂琴, 王建民, 薛乐勋 ( , 50052) [ ] : canstatin cDNA ( E. coli) , : Trizol RNA, RT- PCR canstatin( m canstatin) cDNA, pMD18- T canstatin cDNA pET30a( + ) , E. coli BL21 IPTG : canstatin cDNA 68 bp, 227 , canstatin cDNA 89% , 96% IPTG pET30a( + ) / m canstatin E. coli BL21 : canstatin cDNA, pET30a( + ) / m canstatin E.