柑橘黄龙病对纽荷尔脐橙叶片营养元素及土壤微生物群落的影响.docVIP

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柑橘黄龙病对纽荷尔脐橙叶片营养元素及土壤微生物群落的影响.doc

柑橘黄龙病对纽荷尔脐橙叶片营养元素及土壤微生物群落的影响 管冠1*,李倩磊1,朱婧1,姚锋先1,周高峰1 1. 赣南师范大学国家脐橙工程技术研究中心,中国赣州,341000 基金项目:国家自然科学基金;江西省2011协同创新项目;赣南师范大学校级招标课题(一种生物调理剂对于脐橙果园土壤酸化控制的影响研究);赣州市科技计划项目(用于脐橙果园土壤酸化控制的绿色生物调理剂研究推广与示范)。 作者简介:管冠(1985.11-),男,湖北黄石人,博士,讲师,主要从事土壤生物学研究;E-mail: guanguan_1985@。 摘要:柑橘黄龙病(Citrus Huanglongbing, HLB)堪称柑橘的癌症,严重为害产业健康发展。本研究采用盆栽试验,通过ICP-MS、平板计数法及微平板法测定了不同染病时间的纽荷尔脐橙叶片的矿质营养元素含量、土壤微生物种群数量及微生物功能多样性。研究结果表明,在黄龙病侵染的纽荷尔脐橙病株中,叶片中显著性减少的矿质营养元素多与叶绿素合成及光合系统相关。而土壤微生物群落功能多样性的下降时间段与土壤纤维素分解菌的增加时间段相吻合,表明根系生态系统的破坏多发生于黄龙病侵染后的6-8个月。 Effects of citrus huanglongbing on the nutrient elements in Newhall navel orange leaves and the soil microbial community GUAN Guan*, LI Qian-lei, ZHU Jing, YAO Feng-xian, ZHOU Gao-feng (National Navel Orange Engineering Research Center, Gannan Normal University, Ganzhou China Abstrct: Citrus Huanglongbing (HLB) is the most devastating disease for citrus, which causes great loss in citrus industry. Pot experiments were performed to investigate the effects of citrus huanglongbing on the nutrient elements in ‘Newhall’ navel orange leaves, the number of soil microbes and the soil microbial community functional diversity. The results showed that significant reduction of nutrient elements which in the leaves of the infected ‘Newhall’ is likely to be related to chlorophyll synthesis and photosynthesis system. The reduction of soil microbial community functional diversity and the increasing of soil cellulose decomposing bacteria occur simultaneously, which indicating that the destruction of the root ecosystem would be appeared in 6-8 months after HLB infection. 关键词:柑橘黄龙病;纽荷尔脐橙;营养元素;土壤微生物 Keywords:Citrus Huanglongbing; Newhall navel orange; nutrient elements; soil microbes 柑橘黄龙病(Citrus Huanglongbing, HLB)堪称柑橘的癌症,主要通过木虱在柑橘类植物中传播,严重为害柑橘产业发展[1]。其致病菌 (韧皮部杆菌)属于一种不可培养的微生物,科赫式证病也难以完成[2]。黄龙病在生产上尚不存在有效的治疗措施,其发病症状多样,以红鼻子果和叶片的多种黄化症状最为常见[3]。而黄龙病的侵染不可能改变矿质营养元素本身的生理功能,其对矿质营养元素的吸收转运和分配过程的影响才是黄化症状产生的根源。 在黄龙病侵染柑橘的过程中,存在6-12个月的症状潜伏期,在这段时间中,黄龙病的发生症状并不明显,但其内部营养元素对黄龙病病菌侵染的响应不可忽略[4]。

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