自噬流的检测方法_吕晓希.pdfVIP

Acta Pharmaceutica Sinica 2016, 51 (1): 45−51 45 , * (, , , 100050) : , , , , : ; ; ; ; 1A/1B-3 : R965.2 : A : 0513-4870 (2016) 01-0045-07 New methods to detect autophagic flux LÜ Xiao-xi, HU Zhuo-wei* (State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study, Institute of Meteria Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China) Abstract : Autophagy is a crucial biological process of eukaryotes, which is involved in cell growth, survival and energy metabolism, while the premise of the autophagy function is activated autophagic flux. It has been confirmed that impaired autophagic flux promotes pathogenesis of many chronic inflammatory diseases, especially cancer, neurodegenerative disease and tissue fibrosis, therefore the analysis of autophagic flux state is important for revealing autophagy function and the mechanism of autophagy related diseases. Given that autophagy is a dynamic process with multiple steps, it is very hard to observe the real state of autophagic flux. Summarized here is the novel concept and curre