g25脱盐柱使用指南精编版.pptVIP

Desalting column protocol Mr. Yu Wang Matrix Sephadex™ G-25 Fine, cross-linked dextran Bed volume 53 ml Bed height 100 mm i.d. 26 mm separation of high (Mr 5 000) from low molecular weight substances (Mr 1 000). Storage +4 to +30 °20% ethanol 长期不用后再使用，应该用5CV平衡柱子 （5*60=300ml buffer） Try these conditions first Buffer(35ms/cm): 50 mM phosphate buffer, 0.15 M NaCl, pH 7.0 Flow rate: 20 ml/min at room temperature Sample volume: 15 ml Separations: Elute the column with 106 ml (2 CV) of buffer Equilibration is not necessary between runs with the same buffer. (去除气泡）Reverse the flow direction and pum