野油菜黄单胞菌hpa2基因的突变和功能分析_姜伯乐.pdfVIP

27 　 3 2008 9 Vol.27, No.3 Journal of Guangxi Agric.and Biol.Science Sept., 2008 :1008-34 4 (2008)03-020 -0 hp a2 1, 2 1 3 1 1 姜伯乐 , 蒋国凤, 黄佩芳 , 杭小红, 荆龙, 1 1, 2 1, 2 杨艳 , 何勇强 , 唐纪良 (1　 , 530005; 2　, 530005; 3　 , 530005) :pK18mob X cc 8004 hp a2 , PK3001。 , hp a2 PK3001 , 。 g us RT-PCR hp a2 , , hp a2 , hrp G、 hrpX 。 :;hp a2; :Q344 　　　:A Mutagenesis and functional characterization of hp a2 gene in X anthomonas camp estris pv.camp estris 1,2 1 3 1 1 JIANG Bo-le , JIANG Guo-feng , H UANG Pei-fang , HANG Xiao-hong , JING Long , 1 1,2 1,2 YANG Yan , HE Yong-qiang , TANG Ji-liang (1　College of Life Science and Technology , Guangxi University , Nanning 530005, China; 2　Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Nanning 530005, China; 3　College of Agriculture, Guangxi University, Nanning 530005, China) Abstract:Mutant PK3001 w as generated by the hp a2 gene of X anthomonas campestris pv. camp estris (X cc)mutated w ith suicide plasmid pK 18mob.The pathogenicity and hypersensitive reaction (H R)of mutant PK3001 w ere determined.The hp a2 mutant PK3001 didn t affect the virulence significantly but lost H R completely.The expression and regulation of the hp a2 gene w ere analyzed through gus report gene and RT-PCR.The results showed that the hp a2 gene w as highly expressed in MMX and positively regulated by hrp G and hrp X. Key words:X anthomonas camp est