Virusbindingandentry病毒结合和进入.pptVIP

Clathrin vs. non-clathrin internalization Most viruses were originally assumed to use clathrin as a route into the cell Used by SFV, VSV, adenovirus etc Other routes of entry exist and can be used Caveolae (as used by SV40) are the best characterized) Influenza and rotavirus are other examples In most cases non-clathrin pathways are ill-defined Dynamin is a GTPase that acts to “sever” the necks of the endocytic vesicle It is not specific to clathrin-coated vesicles Dominant-negative mutant (K44A) inhibits endocytosis Eps15 binds to AP-2, the clathrin adaptor protein It is specific to clat