人参总皂甙对造血生长因子活性及其mRNA表达的影响.docVIP

???? 人参总皂甙对造血生长因子活性?及其mRNA表达的影响* ???? ????【摘要】　目的　探讨人参“补气生血”的现代生物学机理。　方法　采用核酸分子原位杂交和造血生长因子生物活性检测等技术，研究人参总皂甙(TSPG)对造血生长因子活性及其mRNA表达的影响。　结果　TSPG能显著促进小鼠骨髓基质细胞(BMSC)、腹腔巨噬细胞(PMφ)和脾细胞(SPC)的粒-巨噬细胞集落刺激因子(GM-CSF)及白细胞介素-6(IL-6)mRNA的表达，经TSPG诱导制备的BMSC、PMφ和SPC条件培养上清液含有较高的爆式红系集落刺激活性(BPA)、粒-巨噬细胞集落刺激活性(GM-CSA)和巨核细胞集落刺激活性(MK-CSA)。　结论　TSPG促进血细胞生成的机理与其诱导造血生长因子的基因表达有密切关系。　　【关键词】　人参总皂甙　造血生长因子　粒-巨噬细胞集落刺激因子　白细胞介素-6　基因表达　原位杂交 EFFECT OF TOTAL SAPONINS OF PANAX GINSENGON THE BIOACTIVITY AND mRNA EXPRESSION OFHEMATOPOIETIC GROWTH FACTORS Wang Yong, Wang Shali, Wang Yaping△,Jiang Rong, Zhang Shushen(Department of Histology and Embryology, Chongqing University of Medical Sciences, Chongqing) 　　【Abstract】 Objective Hematopoietic growth factors (HGF) play a very important role in hematopoietic regulation. Saponins is known as of the main effective fractions in Chinese traditional medicine Panax Ginseng. Our previous study has showed that total saponins of panax ginseng(TSPG) could stimulate hematopoiesis significantly. The present study investigate the effect of TSPG on the bioactivity and mRNA expression of HGF for clarifying the hematonic mechanism of Panax Ginseng. Methods The techniques of nucleic acid in situ hybridization, culture of hematopoietic progenitor cells ex vivo and bioassay of HGF were used. Results TSPG could markedly enhance bone marrow stromal cell (BMSC), peritoneal macrophage(PMφ) and splenic cell (SPC) to express GM-CSF mRNA and IL-6 mRNA. The BMSC, PMφ and SPC conditioned media, prepared by treating these cell with TSPG (20mg/L) ex vitro, could obviously promote proliferation and differentiation of BFU-E, CFU-GM and CFU-MK. These mean that much higher burst promoting activity (BPA), granulocyte and macrophage colony-stimulating activity (GM-CSA) and megakaryocyte colony-stimulating activity (MK-CSF) exist in TSPG induced conditioned media. Conclusion TSPG may regulate hematopoiesis by activating GM-CSF and IL-6 mRNA expression of BMSC. Mφ and lymphocyte in hematopoi