一种非手术性小鼠胚胎移植技术.pdf

440 生 物 工 程 学 报 段新崇 等/一种非手术性小鼠胚胎移植技术 Chinese Journal of Biotechnology /cjbcn April 25, 2016, 32(4): 440−446 DOI: 10.13345/j.cjb.150333 ©2016 Chin J Biotech, All rights reserved 动物及兽医生物技术 1 1 2 3 1 段新崇 ，李阳 ，邸科前 ，黄勇 ，李相运 1 071001 2 071001 3 402460 , , , . . , 2016, 32(4): 440–446. Duan XC, Li Y, Di KQ, et al. A nonsurgical embryo transfer technique in mice. Chin J Biotech, 2016, 32(4): 440–446. 摘 要: 为了提高非手术性小鼠胚胎移植技术的成功率，利用塑料移植导管模拟非手术胚胎移植过程，通过 观察染料在子宫角的分布而评估胚胎移植效果，并在此基础上将自然妊娠3.5 d 的小鼠囊胚经子宫颈移植受体 小鼠。结果表明：将CD-1 小鼠囊胚移植假孕2.5 d 小鼠单侧子宫角，平均70.9% 的胚胎能够发育至成活新生 仔鼠，建立了高效非手术性小鼠胚胎移植技术。该方法简便快捷、不易污染、费用低，无需专业的手术器械， 且符合实验动物伦理原则，完全可以取代手术法胚胎移植技术，更重要的是，它为人类和其他大动物的胚胎移 植提供了研究模型。 : 小鼠，非手术性胚胎移植，囊胚，子宫损伤，胚胎丢失 A nonsurgical embryo transfer technique in mice 1 1 2 3 1 Xinchong Duan , Yang Li , Keqian Di , Yong Huang , and Xiangyun Li 1 College of Animal Science and Technology, Hebei Agricultural University , Baoding 071001, Hebei , China 2 Centre of Laboratory Animal Faculty of Medicine, Hebei University , Baoding 071001, Hebei , China 3 Institute of Animal Husbandry Engineering , Chongqing Academy of Animal Sciences, Chongqing 402460, China Abstract: Although efficient nonsurgical transfer of embryos in mice would provide many advantages over a surgical method, the low success rate of nonsurgical transfer has hampered its acceptance and use. Here, a plastic catheter was used Received: July 20, 2015; Accepted: October 10