分光光度法测定浮游植物叶绿素a的比较研究.docVIP

收稿日期:2005204220基金项目:教育部“211工程”重点学科建设项目及江苏省科技项目作者简介:李振国(1980-,男,山东临清人,硕士. 的浓度分别达到了200ng ΠL 和130ng ΠL 。参考文献: [1]Medsker L L ,Jenkins D and Thomas J F.Odorous com pounds in natural water :22ex o 2hydroxy 222methyl 2bornane ,the major odorous com pound produced by several actinomycetes [J ].Environ.Sci.T ech.1969,3:476-477. [2]王占生,刘文君.微污染水源饮用水处理[M].北京: 中国建筑工业出版社,1999:174-184. [3]C onte E D ,C onway S C ,M iller D W ,et al . Determination of methylis oborneol in channel catfish pond water by s olid phase extraction followed by gas chromatography 2mass spectrometry[J ].Water Research. 1996,30(9:2125-2127. [4]Palmentier J P F P ,T aguchi V Y S ,Jenkins W D ,et al . Determination of geosmin and 22methylis oborneol in water using is otope dilution high res olution mass spectrometry [J ].Water Research.1998,32(2:287-294.[5]黎雯,徐盈,吴文中,等.水体中异味化合物定量测 定的一种有效方法[J ].分析测试技术与仪器.1998,4(2:84-90. [6]Y aqi C ,G uibin J ,Jing fu L ,et al .Multiwalled carbon nanotubes as a s olid 2phase extraction ads orbent for the determination of bisphenol A ,42n 2nonyphenol ,and 42tret 2octylphonol[J ].Anal.Chem.2003,75(10:2517-2521. 分光光度法测定浮游植物叶绿素a 的比较研究 李振国1 ,卢军2 ,王国祥2 ,葛绪广 1 (1.南京师范大学地理科学学院,江苏南京　210097;2.南京农业大学资源与环境科学学院,江苏南京　210095 摘　要:对浮游植物叶绿素a 测定方法中的国家标准方法丙酮三色法与目前国际上使用的方法乙醇单色法进性了比较,实验材料使用了实验室培养的栅藻,避免了藻类种类对于测定结果的影响。实验结果显示,乙醇法的萃取率高于丙酮法,但由于乙醇法实验条件的难以控制,其结果的方差较大;两种方法的测定结果间的相关性很好,相关系数为 019998,因此得出两种方法测定结果间的换算公式为Chla 乙醇=11658Chla 丙酮-51821。还研究了乙酸滤膜的溶解对于测 定结果的影响,结果表明乙酸滤膜的溶解未对测定结果造成明显的影响。关键词:叶绿素a ;丙酮萃取法;热乙醇萃取法 中图分类号:X 832　　　文献标识码:A 　　　文章编号:100226002(2006022******* Comp arison of Measurement of Phytoplankton Chlorophyll 2a Concentration by Spectrophotometry LI Zhen 2guo 1 ,et al 　(School of G eography Science ,Nanjing N ormal University ,Nanjing 210097,China Abstract :Chlorophyll 2a content is widely used as an indicator of the quality of freshwater bodies.Chlorophyll 2a contents of cultures of the chlorophyte Sencedesmus subspicatus determined by means of the 90%hot ethanol and the 90%acetone in this study