植物组织中有机酸的提取方法比较.docVIP

植物组织中有机酸的提取方法比较 董园园,董彩霞,卢颖林,缪辰,沈其荣 3 (南京农业大学资源与环境科学学院,江苏南京210095 摘要:以番茄果实和不结球白菜叶片为试验材料,用蒸馏水(水提、80%乙醇旋转蒸发浓缩(醇提、蒸馏水冷冻干燥浓缩(水提冻干分别提取有机酸,采用高效液相色谱法测定。结果表明:3种提取方法下番茄果实中富马酸、丁二酸含量无显著性差异,草酸、酒石酸、苹果酸、草酰乙酸含量均以水提最高,而丙酮酸和柠檬酸以醇提含量最高;不同提取方法对不结球白菜叶片中草酸、酒石酸、富马酸含量无影响,其他几种有机酸(除草酰乙酸外均以水提和水提冻干含量高于醇提,其中,水提法和水提冻干法提取丙酮酸、苹果酸、丁二酸的含量无显著差异,而柠檬酸含量以水提冻干法最高。综合考虑试验步骤和结果等各方面因素发现,蒸馏水直接提取法既能有效保证有机酸的提取量,又能大大简化试验步骤。 关键词:高效液相色谱;植物组织;果实;有机酸 中图分类号:O658　　　文献标识码:A 　　　文章编号200504014004 Study on the methods of extracti on organ i c aci ds fro m di fferent pl ant tissue by HP LC DONG Yuan 2yuan,DONG Cai 2xia,LU Ying 2lin,M I A O Chen,SHEN Q i 2r ong 3 (College of Res ources and Envir on mental Sciences,Nanjing Agricultural University,Nanjing 210095,China Abstract:I n this experi m ent,organic acids were extracted fr om t omat o fruit and Chinese cabbage leaves by different extracti on methods,such as by distilled water directly (W ,by 80%ethanol and then evaporati on for concentrati on (E ,and by distilled water f or extracti on and then fr ozen 2evaporati on concentrati on (W F and then deter m inated by HP LC .The results obtained are lis 2ted as f oll ows . I n ter m s of t omat o fruits,the extracted a mounts of fu marate and succinate were not influenced by extracti on meth 2 ods,while the a mounts of oxalic acid,tartaric acid,malate and oxal oacetate extracted byW method were significantly higher than those extracted by the other t w o methods .E method could extract the highest amount of pyruvate and citrate .For Chinese cabbage leaves,no significant differences were found in oxalic acid,tartaric acid and fu marate extracted by the three extracti on methods,whereas the a mount of the other organic acids (excep t for oxal oacetate extracted by W and W F method were significantly higher than those extracted by E method .There were no significant difference of pyruvate,malate,and succinate extracted byW and W F method while citrate amount extracted by W F was higher than that by W.Therefore,distill