球形红细菌厌氧降解邻二氯苯及其机理研究.PDFVIP

微生物学通报 MAY 20, 2008, 35(5): 666~673 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 研究报告 球形红细菌厌氧降解邻二氯苯及其机理研究 1,2 2, 3* 2 1 王玉芬 张肇铭 胡筱敏 贡 俊 (1. 030006) (2. 110004) (3. 030006) 摘 要: 研究分析光合细菌球形红细菌在厌氧光照条件下降解邻二氯苯的条件和机理。结果表明, 在厌氧光照条件下球形红细菌的最佳生长和对邻二氯苯的最佳降解条件为：pH 7.0, 温度为30 ℃, 接种量 10 ％。在最佳条件下, 邻二氯苯的去除率可达 90 ％以上; 其降解中间产物主要有氯苯、4- 羟基苯甲酸; 根据降解产物的分析, 推断球形红细菌降解邻二氯苯的机理主要是按照先脱掉一个 氯原子生成氯苯, 然后氯苯进一步脱氯并通过4-羟基苯甲酸的代谢途径开环进行。 关键词: 光合细菌, 球形红细菌, 厌氧降解, 邻二氯苯 Study on Mechanisms and Anaerobic Degradation of 1,2-Dichlorobenzene by Rhodobacter sphaeroides WANG Yu-Fen1,2 ZHANG Zhao-Ming2,3* HU Xiao-Min2 GONG Jun1 (1. Department of Environmental Economics, Shanxi University of Finance and Economics, Taiyuan 030006) (2. School of Resource Environment and Civil Engineering, Northeastern University, Shenyang 110004) (3. College of Life Science and Technology, Shanxi University, Taiyuan 030006) Abstract: The influences of several factors on the growth and removal for 1,2-Dichlorobenzene of Rhodo- bacter sphaeroidcs under phototrophic (anaerobic in light) condition were analyzed in this study. The results demonstrated that the optimum conditions for the growth of the strain and the removal rate for 1,2-Dichlorobenzene were pH 7.0, temperature at 30℃ and inoculation amount of 10% under phototrophic conditionThe removal rate for 1,2-Dichlorobenzene reached 90%under the optimal cond