山茶油脂肪酸甲酯化条件研究.docVIP

山茶油脂肪酸甲酯化条件研究 唐　芳1,李小元1,吴卫国1,刘军鸽2 (1. 湖南农业大学食品科技学院,食品科学与生物技术湖南省重点试验室,　湖南长沙　410128; 2. 湖南农业大学分析测试中心,　湖南长沙　410128摘　要:油脂甲酯法是应用气相色谱法测定油脂脂肪酸组成一个必不可少预处理工序。该文报道 对比研究KOH―甲醇溶液法、H 2SO 4―甲醇溶液法对山茶油进行甲酯化效果,着重探讨振荡温度、振荡时间、催化剂用量等因素对山茶油甲酯化效果影响规律,通过气相色谱法测定油酸峰面积及油酸甲酯转化率为考核指标,优化KOH―甲醇溶液法和H 2SO 4―甲醇溶液法甲酯化条件,并提出酸碱结合甲酯化新方法,研究结果表明,采用酸碱结合法对山茶油进行甲酯化效果优于KOH―甲醇溶液法和H 2SO 4―甲醇溶液法,对应用气相色谱法测定油脂脂肪酸组成具有实际指导意义。关键词:山茶油;甲酯化;KOH―甲醇溶液法;H 2SO 4―甲醇溶液法;酸碱结合法 Study on methylesterification conditions of fatty acid in camellia oil TANG Fang 1,LI Xiao-yuan 1,WU Wei-guo 1,LIU Jun- ge 2 (1. College of Food Science Technology of Hunan Agricultural University,Food Science Biotechnology of Key Laboratory of Hunan Province,Changsha 410128,China;2. Center of Analytic Testing of Hunan Agricultural University,Changsha 410128,ChinaAbstract:The methylesterification method of oils was an essential pretreatment process for the determination of fatty acid composition in oils by gas chromatography.The paper studied the effects of methylesterification in camellia oil through the comparation of KOH –methanol solution method,H 2SO 4–methanol solution method;and explored the rule that the oscillations of temperature and time,the contents of catalyst and other factors would impacted on the effect of methylesterification in camellia oil.With peak area of oleic acid gas chromatography determined and the efficiency of oleic acid methyl ester as an indicators for the assessment,it optimized the conditions of methylesterification of KOH –methanol solution method,and H 2SO 4–methanol solution method,and made a new method of methylesterification of acid –alkali combination.The results on study indicated that acid –alkali combination method for the effect of methylesterification in camellia oil was superior to KOH –methanol solution method and H 2SO 4–methanol solution method for that,and that it had a significance of the practical guide to determine the composition of fatty acid in olein by gas chromatography.Key words:camellia oil;met