肿瘤标志物检测工作临床意义和诊疗价值.pptVIP

肿瘤标志物检测工作临床意义和诊疗价值.ppt

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癌症类型     敏感性   特异性 研究单位 肺癌 98% 97% 伊利沙白女皇医院 结肠癌 83% 92% 浙江大学 乳腺癌 85% 91% 霍普金斯医学院、美国国立癌症研究所 肝癌 91% 89% 浙江大学,香港中文大学 前列腺癌 85% 85% 维吉尼亚前列腺病研究中心 卵巢癌 82% 98%               霍普金斯医学院,FDA 前景光明 seldi在许多疾病的诊断特别在肿瘤早期准确诊断上有着极其光明的应用前景。 美国科学顾问委员会的300多位从事生命科学或医疗一线的研究者普遍认为飞行质谱是一个极有前途的应用技术,它将会大大改变目前如肿瘤、心血管病等诊断准确性落后的情况,将给诊断学带来一场革命或突破,前景美好。 架起我们友谊交流的桥梁 欢迎大家来信 Email: Ranweigd@ 通讯地址:广州市中山二路58号:中山大学附属第一医院预防保健体检中心&广东省健康体检医学会 收 邮编:510080 谢谢 * * The SELDI process graphically looks like this. 1microliter of Crude biological sample added to the chip and the proteins of interest are captured on the appropriate chemical, biochemical or biological affinity capture surface; then, the unwanted sample material is washed away. A laser is used to read the retained proteins which are desorbed and ionized by multiple pulses of laser and detected. The molecular weight of each protein is deduced by the software and displayed as shown. The homogeneous nature of the surface provides excellent quantitative results. Retained proteins can be further processed directly on the chip for sequence, post translational modification, protein interaction studies, or higher resolution protein expression analysis. * The ProteinChip Array containing retained proteins and added EAM is inserted into the Protein Reader for mass spectrometric analysis. The surface of the biochip is pulsed with a UV laser leading to excitation of the EAM and energy transfer to associated analytes. Proteins are desorbed and ionized into a gas phase and accelerate through a voltage gradient before drifting down a flight tube and striking a detector. The time-of-flight of the molecule is proportional to its mass divided by its charge. Typically singly charged ions are formed giving a mass read-out in Daltons. Occasionally, with more concentrated analytes there is the production of doubly charged species that have an artificial mass that is half the mass of the singly charged species. The result is an

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