拉曼光谱基本原理图解.pptVIP

* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * The confocality of a Raman instrument can be demonstrated by moving a sample for Raman analysis through the microscope best white light focus. A measure of the Raman intensity as a function of distance from best white light focus shows the efficiency of collecting out of plane light. An excellent material for testing confocality is Ge. Ge was chosen because the sample is not transparent, and all Raman light must originate from the surface. This testing arrangement (use of an opaque sample) eliminates the complication of co