细胞信号转导和肿瘤的课件.ppt

信号转导的研究方法与工具 一、蛋白质磷酸化状态的检测 1、免疫印迹 (phospho-protein specific antibodies) 2、免疫沉淀 (protein-specific antibody + phospho-AA antibody 3、流式细胞仪分析 4、Luminex分析 二、信号转导分子过度表达或过度激活 1、Overexpression by gene transduction 2、Constitutively activated mutants 三、基因转录活性测定 1、Electrophoretic mobility shift analysis (EMSA) ２、Reporter gene expression detection 四、信号转导分子的表达或活性抑制 1、Anti-sense 2、RNAi 3、Gene knock-out 4、Dominant negative mutants Ligand-binding site Phosphorylation site Docking site Protein-protein binding site DNA binding site 5、Small-molecule inhibitors：e.g., tyrosine kinase inhibitor (TKi) 6、Inhibitory