液质阐述条件的优化策略课件.pptVIP

液质分析条件的优化策略（简化板）;液相色谱与液质联用仪使用的要点;质量校正的正确（Myoglobin校正）;质量校正的正确（Myoglobin校正）;质量校正的正确（CsI校正）的肽测定;质量校正的正确（CsI校正）的蛋白测定;质量校正的关键点;合适的液相色谱平台;合适的液相色谱柱;LC/MS Flow Injection Analysis of Peptides and Proteins by Reversed-Phase HPLC;;Volatile buffer – minimize instrument downtime Buffer concentration: High ? ion suppression decreases ESI sensitivity Low ? system adequately buffered? pH range permitted by stationary phase Methanol or acetonitrile Start with acetonitrile ;Useful pH Ranges for Volatile Buffers;Effect of Buffer on Analyte Response;Mobile Phase pH effect on ESI;Effect of Mobile Phase pH on