实验三土壤中细菌总数的测定教学提纲.pptVIP

实验三 土壤中细菌总数的测定 一 教学要求 通过土壤中细菌总数的测定，了解微生物数量的测定方法，掌握平板菌落计数的原理和方法 二 实验原理 1、测定微生物生长的方法 Direct methods Direct counting，Coulter counter Indirect methods Viable Cell methods，Membrane filtration ， turbidity methods，most probable number(MPN) ,Detect chemical products or metabolic activity Alternative methods for viable cell counts Colony-Forming Units (CFUs) However, keep in mind that if the organism normally forms multiple cell arrangements, such as chains, the colony-forming unit may consist of a chain of bacteria rather than a single bacterium. In addition, some of the bact