金丝草多糖的抗氧化性及还原能力的研究.pdfVIP

Journal of Organic Chemistry Research 有机化学研究, 2015, 3, 16-24 Published Online March 2015 in Hans. /journal/jocr /10.12677/jocr.2015.31003 The Study of Antioxidation and Reducing Ability of Polysaccharides from Pogonatherum crinitum Fenling Li, Hanquan Cai, Lisheng Chen Hanshan Normal University, Chaozhou Guandong Email: lfl8832@126.com rd rd th Received: Jan. 23 , 2015; accepted: Feb. 3 , 2015; published: Feb. 10 , 2015 Copyright © 2015 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract The ultrasonic extraction method was used to extract the polysaccharide from Pogonatherum cri- nitum. The clearing hydroxyl radical ( OH ) and the superoxide anion ( O−) activity of purified 2 polysaccharide were operated to evaluate polysaccharide in vitro antioxidant activity; hydroxyl − radical ( OH ) was produced by Fenton reaction, and superoxide anion ( O2 ) was produced by pyrogallol autoxidation. The spectrophotometric method was used to determine the sodium ni- trite removal ability of the extracted polysaccharide solution of different concentrations. The re- sults showed that the Pogonatherum crinitum polysaccharide has good scavenging ability not only on superoxide anion and hydroxyl radical, but also on sodium nitrite. Keywords Pogonatherum crinitum, Polysaccharide, Ultrasonic, Hydroxyl Radical ( OH ), Superoxide Anion ( O−)